客户简介:
中南民族大学位于湖北省武汉市,是中华人民共和国国家民族事务委员会直属高校,为中国“少数民族骨干计划”资格高校、全国深化创新创业教育改革示范高校、中国高校行星科学联盟、湖北省“国内一流大学建设高校”。
实验目的:
检测藻细胞内总蛋白含量和抗氧化酶活性
实验材料和器具:
样品:微囊藻7806 (Microcystis aeruginosa PCC 7806)
工具:小美超微量超声波细胞破碎仪(XM-650T)
耗材:10ml离心管
实验步骤:
1.准备小美超声波破碎仪,配加长变幅杆
2.将探头放入准备好的样品中处理中
3.超声条件设置
4.具体实验流程:取20ml集胞藻6803藻液(OD0.6-0.8),常温离心,弃上清。沉淀加2ml的tris-HCl(40mM, PH8.0)及20ul的PMSF(100mM),超声处理。超声后,4度离心,取上清转移至新的离心管,用于蛋白定量和抗氧化酶活性检测。
注意:
1、每次破碎样品时尽量避免探头贴壁,也可根据客户自己操作调节
2、由于连续超声破碎,会使样品温度升高,需要客户冰浴处理
实验过程、结果图示:
XM-650T
图1破碎前,图2破碎后
此款仪器为上海净信实业发展有限公司旗下小美超声生产的全新超声波破碎仪一体机,是一种特殊的、快速的、高效率的一体机破碎系统。可以在较短的时间内完成对各种样品的研磨、粉碎、混合及细胞破壁,以保留样品活性。
产品简介:
1、药物提取,细胞,细菌,病毒组织破碎。如细胞内含物的萃取。
2、物质颗粒的分散、匀质,及乳化。如纳米材料的分散。
3、加速溶解,加速化学反应。如用于化学合成,此超声设备配隔音箱支架、专用隔音箱、低温系统。
4、超声破碎是声化学设备的一种应用,可用于植物提取,水处理、固液系分散、液体中颗粒的解团聚、促进固液反应等效果,使较大粒径的聚集体分散为较小的颗粒。稳定化指保证粉体颗粒在液体中保持长期的均匀分散等。
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